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海洋性細菌SMM-7株のホルムアルデヒド減少能に関する基礎研究

石井, 洋; 前田, 孝史; 井上, 亮一; 齋藤, 寛, 2012.03.20. -- (東海大学紀要. 海洋学部. 9巻3号). w. <TC10000041>
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9-3-43-48-2011.pdf

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タイトル 海洋性細菌SMM-7株のホルムアルデヒド減少能に関する基礎研究
タイトル(その他) Study on reduction of formaldehyde in SMM-7 marine bacterial strain
作成者 石井, 洋
イシイ, ヒロシ
Ishii, Hiroshi
作成者 前田, 孝史
マエダ, タカフミ
Maeda, Takafumi
作成者 井上, 亮一
イノウエ, リョウイチ
Inoue, Ryoichi
作成者 齋藤, 寛
サイトウ, ヒロシ
Saito, Hiroshi
公開者 東海大学海洋学部
公開者 The School of Marine Science and Technology, Tokai University
公開者 東海大学出版会
掲載巻号 東海大学紀要. 海洋学部. 9巻3号 <TB00000033>
ISSN 13487620
雑誌名 東海大学紀要. 海洋学部
巻次等 9
3
開始終了ページ 43-48
発行日 2012.03.20
内容記述 Based on our results on the growth of marine bacterial strain, SMM-7 in nutrient broth medium with the high con-centration (750 ppm) formaldehyde (FAD), as presented in another paper, we inferred that this bacterial strain is capable of bioremediation in ocean. There is a possibility that the bacteria possess enzyme against the effect of FAD. In this study, we investigated the cellular location of such enzyme to understand the degradation pathway of FAD in the SMM-7 strain.
内容記述 The SMM-7 strain was cultured in nutrient broth medium with FAD, the culture was centrifuged, and the cell supernatant and subnatant was recovered. The cell subnatant was treated with phosphate buffer (pH7.0) 3 times, and recovered after 3rd treatment. After treatment, the cell homogenate was centrifuged, supernatant was filtered through Millipore filter (0.45μm), and the cell extract was recovered. Finally, 10 ppm FAD was added to cell supernatant, subnatant with phosphate buffer and cell extract, and conc0entration of FAD was measured using Nash method. Furthermore, heat treatment was applied to the cell supernatant and cell extract. Reduction of FAD was clearly noticed in cell extract. In contrast there was no reduction of FAD in the cell extract after applying the heat treatment. The results indicate 1) that SMM-7 strain might resist to FAD intracellularly 2) that the reduction effect of FAD in the cell could be due to enzyme.
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